Detection of Intranuclear T-bet in T Cells

microbiology
NYU
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 647 anti-T-bet Antibody

Catalog Number:

644803

Clone Number:

4B10

We are interested in detecting T-bet + T cells in lymph nodes and tissues of mice infected with S. aureus.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Lymph node cells

Primary Incubation

3uL/test, 30 min, room temperature

Blocking Agent

FC block

Secondary Incubation

-

Tertiary Incubation

-

Detection

ZE5 Biorad YETI Flow cytometer

Results Summary

Single cell suspensions of draining lymph nodes from S. aureus infected mice were stained (extracellular), fixed, permeabilized, and stained for intranuclear expression of Tbet. flow cytometry gate on left is uninfected mouse and gate on right is S. aureus infected mouse

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use, reliable, repeatable

The Bad

Requires 3uL/test

The Bottom Line

Reliable detection of intranuclear Tbet.

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