Detection of Intracellular Mouse IL-4

April 13, 2020

microbiology
NYU
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse IL-4 antibody

Catalog Number:

504105

Clone Number:

11B11

We are interested in the frequency of IL-4 producing T cells in the lymph node and lung following allergic sensitization.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

mouse medistinal lymph node cells

Primary Incubation

1:100; room temperature; 30 minutes

Blocking Agent

FC block

Secondary Incubation

-

Tertiary Incubation

-

Detection

ZE5 YETI Biorad flow cytometer

Results Summary

Lymph node cells from non-sensitized (left) or allergen-sensitized (right) mice were restimulated in PMA/Ionomycin in the presence of Brefeldin A for detection of IL-4 producing lymphocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to optimize

The Bad

Need to use at 1:100

The Bottom Line

Easy and reliable detection

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