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Alexa Fluor® 700 anti-mouse CD19 Antibody
115528
6D5
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Spleenocytes were stained with AF700 anti-CD19 antibody. Staining was distinguished on a ZE5 YETI flow cytometer.
Flow Cytometry
Mouse splenocytes
1:200 dilution of antibody for 20 minutes at room temperature
None
Flow cytometry
Observed okay separation between pos and neg populations. Eventually switched to APC CY7 for detection
N/A
AF 700 doesn't bleed into a lot of other channels
Not great separation
Okay if used with another B cell marker or if that is the only color you can make work.