Cell Signaling Technology
RBX1 Antibody #4397
4397
Polyclonal
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RBX1 is a small protein, the western blot for it could be tricky. This antibody works relatively well.
Western Blot
HEK293T
1:1000 4 ℃ overnight
5% milk
1:10000, room temperature, 1 hour
None
Fluorescence
Got bands at expected molecular weight, but the signal is not super strong
N/A
Specific signal
Signal not strong
This antibody works fine to detect endogenous RBX1 from mammalian cells. The signal is not very strong, needs to load more total protein to get good signal.