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PE anti-human CD25 Antibody
302606
BC96
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Browse Similar Products: CD25 Flow Cytometry Antibodies
Our lab studies T cells throughout the human body by using various organ tissues and extracting the lymphocyte populations for sorting. One of our sorting gates is based on CD25+ expression and we chose this antibody because it is bright.
Flow Cytometry
Human spleen cells
Cells were stained in 100ul FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS
None
BD Influx
This antibody works well enough to see the CD25+ population.
N/A
Easy to use
Not always a lot of separation
This antibody works well enough for cells with medium amounts of CD25.