Miltenyi Biotec
Anti-human CD62L PE/Vio770
130-114-147
145/15
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This antibody was used to stain for CD62L expression in human peripheral blood. It was chosen because it is a commonly used clone for CD62L, PE/Vio770 is bright and works well with other fluorophores in the staining panel, and Miltenyi has a good reputation for making quality flow cytometry antibodies.
Flow Cytometry
Whole blood
5 ul per test in 100uL staining volume for 15 minutes at room temperature.
FcBlock + 0.2% BSA
N/A
BD LSR II
CD62L staining was excellent (filled histogram) as compared to unstained cells (dotted line).
Works well. Reproducible results.
Will continue to use this antibody.