Staining Peripheral Blood with Anti-CD49d BV605

April 02, 2020

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Anti-human CD49d BV605

Catalog Number:

304324

Clone Number:

9F10

This antibody was used to stain for CD49d expression in human peripheral blood. It was chosen because it is a commonly used clone for CD49d, BV605 is bright and works well with other fluorophores in the staining panel, and Biolegend has a good reputation for making quality flow cytometry antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole blood

Primary Incubation

5 ul per test in 100uL staining volume for 15 minutes at room temperature.

Blocking Agent

FcBlock + 0.2% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

CD49d staining was excellent. Positive cells were fully resolved from negative.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well. Reproducible results. Low price.

The Bad

N/A

The Bottom Line

Will continue to use this antibody.

Share your experience with other scientists. Write a Review! »

Join the discussion