Miltenyi Biotec
Anti-human CD4 VioGreen
130-113-783
VIT4
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This antibody was used to stain for helper T cells in human peripheral blood. It was chosen because it is a commonly used clone for CD4, VioGreen works well with other fluorophores in the staining panel, and Miltenyi has a good reputation for making quality flow cytometry antibodies.
Flow Cytometry
Whole blood
5 ul per test in 100uL staining volume for 15 minutes at room temperature.
FcBlock + 0.2% BSA
N/A
BD LSR II
CD4 staining was excellent (filled histogram) as compared to unstained cells (dotted line).
Works well. Reproducible results.
Will continue to use this antibody.