Expression of JUN B in RAW 264.7 Cells

SCHOOL OF VETERINARY MEDICINE AND BIOMEDICAL SCIENCES
University of Nebraska
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

JUN B

Catalog Number:

SC-73 X

Clone Number:

C-11

Jun-B is a transcription factor that is encoded by the JUN B gene. Transcription factor jun-B is a transcription factor involved in regulating gene activity following the primary growth factor response. Here we performed our study measuring increased levels of JUN B.

Experimental Design and Results Summary

Applications

Western Blot

Sample

RAW 264.7 cells lysate

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skim milk

Secondary Incubation

1:5000 dilution in 5% skim milk prepared in 1X TBST

Tertiary Incubation

N/A

Detection

ECL

Results Summary

We found increased levels of JUN B in disease condition.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

No background

The Bad

None

The Bottom Line

This antibody is very good. Highly recommended.

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