Expression of pAKT in Microglia Cells

April 10, 2020

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

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Company:

Cell Signaling Technology

Product Name:

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb

Catalog Number:

4060

Clone Number:

D9E

BV2 microglia cells were treated with 1µM LPA in the presence or absence of LPAR5 inhibitor (different pre-incubation time periods with the inhibitor). Changes in the expression of pAKT were detected using immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BV2 Microglia cells

Primary Incubation

1:1000, O/N, +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit

Tertiary Incubation

N/A

Detection

ECL plus

Results Summary

Treatment with 1µM LPA increased the phosphorylation of AKT in BV2 cells. This effect was abrogated in the presence of LPAR5 inhibitor (TCLPA5).

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Works very well

The Bad

N/A

The Bottom Line

A very good antibody that gives reproducible results.

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