Cell Signaling Technology
Phospho-SAPK/JNK (Thr183/Tyr185) Antibody
9251
Polyclonal
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BV2 microglia cells were treated with 1µM LPA in the presence or absence of LPAR5 inhibitor. Changes in the phosphorylation of JNK were detected using immunoblotting.
Western Blot
BV2 Microglia cells
1:1000, O/N, +4
5% milk
1:5000 anti rabbit
N/A
ECL plus
Treatment with 1µM LPA increased the expression of pJNK in BV2 cells. This effect was abrogated in the presence of LPAR5 inhibitor (TCLPA5).
Works as expected
Some times the bands appear to be not so strong and needs longer exposure time
Generally a good and reliable antibody.