Expression of p38 in Microglia Cells

April 07, 2020

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

p38 MAPK Antibody

Catalog Number:

9212

Clone Number:

Polyclonal

BV2 microglia cells were treated with 1µM LPA in the presence or absence of LPAR5 inhibitor. Changes in the expression of total p38 were detected using immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BV2 Microglia cells

Primary Incubation

1:1000, O/N, +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit

Tertiary Incubation

N/A

Detection

ECL

Results Summary

As expected, no changes were observed in the expression of p38 between different samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works as expected

The Bad

N/A

The Bottom Line

Very good and reliable antibody.

Share your experience with other scientists. Write a Review! »

Join the discussion