Expression of pp38 in Microglia Cells

April 06, 2020

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

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Company:

Cell Signaling Technology

Product Name:

Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb

Catalog Number:

9215

Clone Number:

3D7

BV2 microglia cells were treated with 1µM LPA in the presence or absence of LPAR5 inhibitor. The inhibitor was tested for its efficacy through different pre-incubation periods. Changes in the expression of pp38 were detected using immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BV2 Microglia cells

Primary Incubation

1:1000, O/N, +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit

Tertiary Incubation

N/A

Detection

ECL plus

Results Summary

Treatment with 1µM LPA increased the expression of pp38 in BV2 cells. This effect was abrogated in the presence of LPAR5 inhibitor (TCLPA5).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works as expected

The Bad

N/A

The Bottom Line

A reliable and good Ab for detecting changes in pp38 expression.

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