Expression of ERK1/2 in Microglia Cells

April 03, 2020

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

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Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb

Catalog Number:

4695

Clone Number:

137F5

BV2 microglia cells were treated with 1µM LPA in the presence or absence of LPAR5 inhibitor. Changes in the expression of total Erk1/2 were detected using immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BV2 Microglia cells

Primary Incubation

1:2000, O/N, +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit

Tertiary Incubation

N/A

Detection

ECL

Results Summary

No changes were observed in the expression of ERK1/2 between different samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works very well

The Bad

Don't strip the same plot as the one used for phospho ERK1/2 detection. Some bands do not appear..

The Bottom Line

A very good antibody.

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