Detection of pNrf2 in Primary Murine Microglia Cells

March 02, 2020

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Recombinant Anti-Nrf2 (phospho S40) antibody [EP1809Y]

Catalog Number:

ab76026

Clone Number:

EP1809Y

Microglia cells were treated with 5µM for different time points. The purpose of the experiments was to detect whether LPA induces changes in the expression of pNrf2.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Primary murine microglia cells

Primary Incubation

1:500, O/N, +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit

Tertiary Incubation

N/A

Detection

ECL plus

Results Summary

Changes in the activation of Nrf2 were detected at 2 and 8 hours after LPA treatment.

DOI or PMID #

N/A

Additional Notes

Strangely it was not possible to detect the expression in some plots. After striping and re incubation with the antibody, the detection was successful.

Related Categories

Image Gallery

Summary

The Good

Works very well

The Bad

N/A

The Bottom Line

A good Ab for the detection of phospho-Nrf2.

Share your experience with other scientists. Write a Review! »

Join the discussion