Detection of IL-6 in Microglia

February 24, 2020

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

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Company:

Abcam

Product Name:

Anti-IL-6 antibody

Catalog Number:

ab6672

Clone Number:

Polyclonal

A human microglia cell line was used in our experiments. Microglia were treated with 1µM LPA for 2 time points and the levels of IL-6 expression were detected with WB.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human microglia cells

Primary Incubation

1:500, O/N, +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit

Tertiary Incubation

N/A

Detection

ECL plus

Results Summary

LPA treatment induces increase in IL-6 expression after 8h.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works as expected

The Bad

N/A

The Bottom Line

Easy-to-use Ab and works quite well.

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