Detection of iNos in Microglia

January 29, 2020

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-iNOS antibody

Catalog Number:

ab3523

Clone Number:

Polyclonal

Microglia cells were treated for 8 and 24 hours with 1µM LPA in order to detect changes in iNos expression. The antibody works quite well.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Microglia cells

Primary Incubation

1:500, O/N

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit

Tertiary Incubation

N/A

Detection

ECL plus

Results Summary

LPA induces increased iNos expression in microglia cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works quite well

The Bad

N/A

The Bottom Line

A good Ab for iNos detection.

Share your experience with other scientists. Write a Review! »

Join the discussion