Fluorescence Activated Cell Sorting (FACS) with Antibody CD14

SCHOOL OF VETERINARY MEDICINE AND BIOMEDICAL SCIENCES
University of Nebraska
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Novus Biologicals

Product Name:

Antibody

Catalog Number:

NB100-62994APC

Clone Number:

N/A

For analysis of cell populations, the cells must be in a high state of purity. Fluorescence activated cell sorting (FACS) is a superior method in which to obtain pure cell populations. We isolated PBMC cells from calf blood then we used to isolate only macrophage cells using CD14 antibody cell sorting FACS methods. We got good CD14 binding pure macrophage cells using Novus Biologicals CD14 antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Cells

Primary Incubation

1:100

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cell sorting FACS

Results Summary

We isolated PBMC cells from calf blood then we used to seperate only macrophage cells using CD14 antibody cell sorting FACS methods. we got good CD14 binding pure macrophage cells using Novus Biologicals CD14 antibody.

DOI or PMID #

N/A

Additional Notes

Nil

Related Categories

Image Gallery

Summary

The Good

Good antibody to sorting macrophage cells

The Bad

Nil

The Bottom Line

We used Novus Biologicals CD14 antibody to separate only macrophage cells using cell sorting FACS method. I got awesome pure macrophage cells.

Share your experience with other scientists. Write a Review! »

Join the discussion