Detection of Apoptosis by Caspase 3/7 Assay Kit

University of Nebraska
SCHOOL OF VETERINARY MEDICINE AND BIOMEDICAL SCIENCES
Postdoctoral Researcher

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Company:

Promega

Product Name:

Apo-one homogeneous caspase-3/7 assay kit

Catalog Number:

G7790

Caspase-3/7 activated apoptosis, irrespective of the specific death-initiating stimulus, and both proteases are widely considered to coordinate the demolition phase of apoptosis by cleaving a caspase 3/7. we performed here mycobacterium induced apoptosis we got good result activation of caspase 3/7.

Experimental Design and Results Summary

Application

Spectramax molecular device

Starting Material

Cells and mycobacterium

Tips

Choose time point study for caspase 3/7 assay

Results Summary

We got good results of caspase 3/7 activation by using the apo one homogenous caspase 3/7 assay kit

DOI or PMID #

N/A

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Summary

The Good

It's working very good

The Bad

Substrate must be maintained on ICE

The Bottom Line

I am using apo one caspase assay kit and it is a very good product and I got good results.

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