Cell Signaling Technology
Phospho-MYPT1 (Thr696) Antibody
5163
Polyclonal
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MYPT1 is the protein that regulates PP1 function in smooth muscle relaxation. Structural elements amino- and carboxy-terminal to the RVxF motif of MYPT1 are positioned in a way that leads to a pronounced reshaping of the catalytic cleft of PP1, contributing to the increased myosin specificity of this complex.
Western Blot
Cell and mouse tissue lysate samples
1:1000 dilution in 5% skim milk prepared in 1X TBST
5% skimmed milk
1:4000 5% skim milk prepared in 1X TBST
None
ECL
We found a time-dependent increase of P-MYPT1 by chemoattractant.
N/A
No background and very strong signal
This antibody is very specific and the signal is strong. Highly recommended.