Time Course Effect of P-eNOS by Chemoattractant

Cellular/Molecular Biology
Texas Tech Health Sciences Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

P-eNOS

Catalog Number:

9571S

Clone Number:

Polyclonal

Nitric-oxide synthase (NOS)2 is a critical enzyme that converts L-arginine (l-Arg) to l-citrulline and nitric oxide (NO) with the consumption of NADPH. NO is a signaling molecule that promotes vascular smooth muscle relaxation and functions as an endogenous mediator of a wide range of effects in different tissues.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell and mouse tissue lysate samples

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skimmed milk

Secondary Incubation

1:4000 5% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We checked cell lysate and mouse tissue Aortic samples the protein expression of Phosphorylation of eNOS very strong.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody is very specific and the signal is strong. Highly recommended.

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