Cell Signaling Technology
NF-κB p65 (D14E12) XP® Rabbit mAb
8242
D14E12
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Browse Similar Products: NF-KappaB p65 Western Blot Antibodies
We examined the expression of P65 in glioma cell lines in our study. 1.5 million cells were harvested and lysed in RIPA buffer (150uL) (added 10X protease inhibitor cocktail). Kept on ice for 5 minutes and centrifuged at 13000rpm for 10 min. Supernatant was collected and loaded 60uL of lysate on 10% SDS-PAGE.
Western Blot
Glioma stem cell whole cell lysates
1:1000 in 5% BSA, overnight in cold room
5%Milk
1:10000 in 5%milk, room temperature for 1 hour
N/A
Chemi doc, femto reagent
Observed a single specific band corresponding to P65 protein molecular weight at approximately 65KD size by western blot.
Clean blot with specific band without any non-specific bands
Excellent antibody for western blot analysis