Great Anti-Mouse TCR ß Chain Antibody

January 28, 2020

Innate Immunity
DRFZ
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience™

Product Name:

TCR beta Monoclonal Antibody (H57-597), PerCP-Cyanine5.5

Catalog Number:

45-5961-82

Clone Number:

H57-597

The antibody was used to isolate TCRbeta+ cells from murine bone marrow. This antibody is good and sensitive with high resolution.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine bone marrow cells

Primary Incubation

20mins 37oC

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACSymphony, software FACSDiva

Results Summary

Good, high resolution, could distinguish cells expressing the TCR beta chain among bone marrow cells from C57BL/6J mice

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good, sensitive, high resolution

The Bad

No bad things about it

The Bottom Line

I would definitely recommend this antibody

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