Alexa Fluor® 700 Anti-Mouse/Human CD11b Antibody

January 23, 2020

Advanced Diagnostics
UofAlberta
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 700 anti-mouse/human CD11b Antibody

Catalog Number:

101222

Clone Number:

M1/70

This antibody was used for identification of cardiac DC subsets using anti-mouse CD103 and anti-CD11b antibodies. It is specific, but not very bright.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Cardiac cells

Primary Incubation

15 minutes 4°C

Blocking Agent

Anti-CD16/CD32

Secondary Incubation

30 minutes 4C

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Identification of cardiac DC subsets using CD103 and CD11b labeling

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very specific

The Bad

Not very bright

The Bottom Line

This antibody is useful to identify cardiac CD11b DCs, but nor very bright labeling

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