Abcam
Donkey Anti-Goat IgG H&L (HRP)
ab6885
Polyclonal
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Since our samples are murine tissue and cells, we use this antibody for detecting any anti-goat secondary. Our lamin-b happens to be a goat polyclonal therefore we use this as our secondary to detect lamin b as one of our possible loading controls on western.
Western Blot
Murine T cells and muscle cells
1:5000 in 4% BSA
Nonfat dry milk
1:5000 in nonfat dry milk
N/A
ECL
The results have always been great. Our lamin-b does show us two different bands but due to it being a polyclonal, that is probably the issue with the primary than this secondary antibody.
Excellent and consistent
The price is a bit high but luckily we don't have a lot of goat antibodies
This is one of the best on the market despite the price being high