Cell Signaling Technology
Anti-rabbit IgG, HRP-linked Antibody
7074
N/A
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Cell Signaling is one of the most reliable for our lab. Their antibodies are most monoclonal and clean. There is rarely non-specific bands. This anti-rabbit hrp has given us excellent results 90% of the time.
Western Blot
Murine T cells and muscle cells
1:2000 in 4% BSA
Nonfat dry milk
1:2000 to 1:5000 dilution depending on primary antibody
ECL
There has been a few times where we had to increase the concentration of this secondary to 1:2000 in order to detect our primary antibody (usually due to poor quality of the antibody or tissue sample). For tissues, the secondary works very well every time while in-vitro samples might require 1:2000 at times.
Excellent Antibody even at 1:5000 dilution
None
This is a required anti-rabbit antibody for secondary. It works so well for western blots and there is always consistent results.