Thermo Fisher Scientific
pHrodo™ Green STP Ester
P35369
The aim was to detect pHrodo™ Green labeled apoptotic endothelial cells engulfed by skin cells. It worked perfectly.
Phagocytosis
Skin wound cells
Follow the protocol in paper "Miksa M, Komura H, Wu R, Shah KG, Wang P. A novel method to determine the engulfment of apoptotic cells by macrophages using pHrodo succinimidyl ester. J Immunol Methods. 2009 Mar 15;342(1-2):71-7"
pHrodo™ Green labeled endothelial cells were engulfed by skin cells probably phagocytes.
N/A
It is easy to label the apoptotic cell with pHrodo™ Green.
None
It worked better than expected. Recommended.