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PE anti-mouse CD23 Antibody
101608
B3B4
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining follicular B cells, that express CD23.
Flow Cytometry
C57BL/6 spleen
surface staining with anti-CD23 1/3200 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
We can stain CD23 using this antibody. And FO B cells (CD23hi/CD21lo) can be gated. The best signal to noise ratio is at 1/3200 dilution. There is background staining at higher concentrations of anti-CD23.
The antibody works
Background staining
Can be used