Thermo Fisher Scientific
MitoTracker™ Green FM
M7514
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We are interested in studying the mechanism of T cell differentiation through modulating cellular metabolism.
Flow Cytometry
Mouse T cells
Cell number and titration is very critical
Cells were incubated with the dye at 37degree in complete growth media. Cells then were washed 3 times with FACS buffer and immediately run on flow cytometer.
N/A
Very good staining by flow
Cannot be fixed with PFA
Very useful tool to study mitochondrial mass by flow cytometry