Cell Signaling Technology
Bcl-2 (D17C4) Rabbit mAb (Mouse Preferred)
3498
D17C4
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To assess differences in cell death, it's important to check expression levels of anti or pro-apoptotic transcription factors. We routinely analyze BCL-2 levels in our immunoblotting samples when we observe decreases in cell death. BCL-2 is an anti-apoptotic transcription factor that plays a critical role in this process.
Western Blot
Murine T cells
1:2000 in 4% BSA/TBST overnight 4 deg
5% milk/TBST for 1 hour at room temperature
1:5000 anti-Rabbit HRP in 5% milk/TBST for 1 hour RT
None
UVP imager
We can detect differential expression of BCL-2 in our samples at the appropriate size band.
N/A
Don't run the gel to far as it's a smaller protein
Clean antibody with no noisy signal. Can reuse diluted antibody for 20 plus times.
None.
Great antibody to detect BCL-2 expression in lysates.