Time Course Effect of NFKB P65 by Chemoattractant

December 09, 2019

Cellular/Molecular Biology
Texas Tech Health Sciences Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz Biotechnology

Product Name:

NFKB P-65

Catalog Number:

SC-372

Clone Number:

F-6

The role of plasma membrane transporters in cancer is receiving increasing attention in recent years and several transporters for essential nutrients are up-regulated in tumour promoters. Here we tested our experiment time course effect of NFKB P-65 by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell and mouse tissue lysate samples

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skimmed milk

Secondary Incubation

1:5000 5% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

My results we found that time dependent increase of NFKB-p65 by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody is very specific and the signal is strong. Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion