Great Antibody for Assessing Total Ribosomal S6 Protein for Immunoblotting

December 11, 2019

Oncology
Johns Hopkins School of Medicine
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

S6 Ribosomal Protein (5G10) Rabbit mAb

Catalog Number:

2217

Clone Number:

5G10

Our lab studies the role of mTOR signaling in T cells. With immunoblot analysis, we use this antibody to normalize total ribosomalS6 protein compared to pS6 (S240.244) activity.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse T cells

Primary Incubation

1:10000 in 4% BSA in TBST 4 deg overnight

Blocking Agent

5% dry milk in TBST 1 hr minimum at RT

Secondary Incubation

1:5000 anti-Rabbit HRP in 5% milk in TBST 1 hr at RT

Tertiary Incubation

None

Detection

UVP

Results Summary

We are able to detect total ribosomal S6 levels. It's a nice antibody to compare the active state of S6 versus total levels.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Great antibody

The Bad

Cheap since can use a high dilution for detection

The Bottom Line

Great great antibody critical for assessing total S6 levels.

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