Cell Signaling Technology
Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb
2997
C77D7
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Our lab studies the role of cellular signaling in T cells during T cell differentiation and function. One important pathway of interest is the mTOR pathway. With immunoblot analysis, we readily assessed p-PRAS40 (T246) activity since its a critical mediator of mTORC1 signaling in WT T cells upon T cell activation under different conditions.
Western Blot
Murine T cells with stim
1:10000
5% milk in TBST
1:5000 anti-Rabbit HRP in 5% milk TBST
None
UVP Imager
We can readily detect PRAS40 activity via phosphorylation with immunoblotting analysis.
N/A
Dilute appropriately since it's a very strong signal
Robust antibody
Great antibody to assess activity of PRAS40 involved in mTOR signaling via AKT.