BOSTER BIOLOGICAL TECHNOLOGY
Anti-Calbindin Antibody
M03047-2
Polyclonal
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I tried this antibody for Purkinje cell labeling in mice cerebellum using IHC-P. Antigen retrieval - 20min boiling in EDTA based buffer. Permeabilization - 0.3% triton for 15min. Blocking - 10% goat serum for 1hr. Primary antibody - 1:250 overnight at 4 Degree CSecondary antibody - Fluorescence conjugated secondary antibody at 1:250 2hr- RT. I was amazed to see the beautiful dendrites, soma and axons. In my hand, this is the best labeling of Purkinje cells I have ever seen using IHC-P.
Immunohistochemistry
Mice Cerebellum
1:250, overnight at 4 Degree C
10% goat serum
1:250, 2hr at room temperature.
None
Fluorescence
Beautiful labeling of Purkinje cell soma, dendrites and axons.
31618636
N/A
Best antibody I have used till now
Highly recommended for IHC-P.