Easy-to-Use Stripping Buffer for Western Blot from Thermo

Seoul National University
Biology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Restore PLUS Western Blot Stripping Buffer

Catalog Number:

46430

This is a pre-made stripping buffer for WB analysis. It works well for most antibodies.

Experimental Design and Results Summary

Application

WB band stripping after.

Starting Material

HEK293T lysate on PVDF membrane

Tips

I incubate WB membrane with this buffer for 20 min at room temperature

Results Summary

This works well with most antibodies. But some antibodies that show strong band intensity do not get completely stripped off.

DOI or PMID #

N/A

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Summary

The Good

Easy to use

The Bad

Some band signals are not completely removed

The Bottom Line

I keep this buffer on my shelf for easy stripping of WB bands.

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