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Brilliant Violet 421 anti-mouse CD138 (Syndecan-1) Antibody
142508
281-2
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B lineage cells play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. We are interested in elucidating the biology of B lineage cells during autoimmunity. In this experiment, I use anti-CD138 to stain antibody-secreting cells.
Flow Cytometry
Wild-type mouse spleen and bone marrow
Surface staining with anti-CD138 (1/800), CD19, etc (20 min, 4° in the dark)
Clone 2.4G2 (Fc block)
N/A
Flow cytometry, violet laser
I could stain CD138hi plasma cells in the spleen and BM.
The antibody works well
This same antibody used to work at 1/1600 in the past, now it needs to be used at 1/800
Advisable, can be used to obtain a clear population.