Anti-Mouse CD138 with Bright Fluorochrome and Little Spillover

November 20, 2019

Charite Strasse 1
Deutsches Rheuma-Forscungszentrum
Research Scientist

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Company:

BioLegend

Product Name:

Brilliant Violet 421 anti-mouse CD138 (Syndecan-1) Antibody

Catalog Number:

142508

Clone Number:

281-2

B lineage cells play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. We are interested in elucidating the biology of B lineage cells during autoimmunity. In this experiment, I use anti-CD138 to stain antibody-secreting cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Wild-type mouse spleen and bone marrow

Primary Incubation

Surface staining with anti-CD138 (1/800), CD19, etc (20 min, 4° in the dark)

Blocking Agent

Clone 2.4G2 (Fc block)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry, violet laser

Results Summary

I could stain CD138hi plasma cells in the spleen and BM.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

The antibody works well

The Bad

This same antibody used to work at 1/1600 in the past, now it needs to be used at 1/800

The Bottom Line

Advisable, can be used to obtain a clear population.

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