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APC anti-human CD45RA Antibody
304112
HI100
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This antibody has been used to optimise flow cytometry-based immunophenotyping of whole blood samples. The results have not yet been published. Multi-colour antibody panels were designed to identify a number of lymphocyte populations and tested to ensure appropriate compensation was possible. This antibody was used within a ten-colour staining panel for T cells.
Flow Cytometry
Human whole blood
2.5µL APC-CD45RA for 30 minutes at 4°C
FcR blocking reagent, human (Miltenyi Biotec, cat# 130-059-901)
N/A
Beckman Coulter Gallios, Kaluza Software
Clear staining that allowed the distinction of CD45RA+ T cells. Used in combination with CCR7, memory and effector cells can be identified. Titration optimisations showed we could reduce the volume of antibody used with no detriment to the results. Prior to analysing samples, erythrocytes were lysed using 1.5 mL 1xPharmLyse (BD, cat#555899) at room temperature for 30 minutes. This antibody fit well into a ten-colour panel with no compensation issues.
Clear staining of CD45RA+ cells that is useful for FACS analysis.
The antibody has only been tested for flow cytometry.
Easy-to-use antibody pack that provided clear and distinct staining of CD45RA+ T cells.