Using CXCL12 in Cell Migration Assay as an Inducer of Migration

November 12, 2019

Aptose Biosciences
R & D
Senior Research Associate

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Company:

Peprotech

Product Name:

RECOMBINANT HUMAN SDF-1A (CXCL12)

Catalog Number:

AF-300-28A

The human recombinant CXCL12 was used to induce migration in RS4;11 cells. Different FBS concentrations were tested. CXCL12 successfully induced migration in RS4;11 cells.

Experimental Design and Results Summary

Application

Migration Assay

Starting Material

Cells

Tips

Used transwells with 8um pore size

Results Summary

Given 4 hours incubation time, RS4;11 cells migrated towards CXCL12 at 100ng/mL.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy to use, reliable results

The Bad

None

The Bottom Line

The recombinant human CXCL12 is a great reagent for migration experiments.

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