Time Course Effect of Chemoattractant on pp38 Phosphorylation

November 13, 2019

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

p-p38 Antibody

Catalog Number:

sc-7973

Clone Number:

D-8

P38 MAPK play a vital role in many biological processes, including cell adhesion and spreading, cell differentiationand apoptosis. Here we checked the time course effect of chemoattractant on p-p38 phosphorylation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We observed time dependent increase of p38 phosphorylation by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Highly specific and strong signal

The Bad

None

The Bottom Line

This antibody works great for western blot. Highly recommended.

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