Santa Cruz Biotechnology
CXCR-4 Antibody (4G10)
sc-53534
4G10
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The CXCR4 is chemokine, primarily functions as chemoattractants for neutrophils. I have used this antibody to detect expression of CXCR4 in macrophages (J774a.1 cells)
Western Blot
Macrophage cell line
1:500, overnight on shaker in cold room
5% skimmed milk
1:2000 HRP conjugated, 1hr at room temp
N/A
Enhanced chemiluminescence (ECL) method
Results were very good. This antibody at 1:500 dilution worked well.
Washing is very important between the incubation
Very good
None
This is one of the good, sensitive and specific antibody from Santa crutz biotech. It worked for me. If follow the protocol correctly, results will be nice and clear. I recommend this antibody to fellow researchers.