A Good Pacific Blue Anti-Mouse MHCII Antibody from BioLegend

December 11, 2019

Harvard Medical School
Harvard
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Pacific Blue™ anti-mouse I-A/I-E Antibody

Catalog Number:

107620

Clone Number:

M5/114.15.2

I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse tissue single cell suspension

Primary Incubation

1:100 dilution for 1 hour on ice

Blocking Agent

Blocking with 2% BSA and Fc block (1:100)

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

Efficient MHCII expression detection on mouse immune cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good antibody

The Bad

Weak fluorophore

The Bottom Line

Good antibody for efficient detection of MHCII on mice immune cells.

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