Santa Cruz Biotechnology Inc
Ref-1 Antibody (15)
sc-136057
15
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: Ref-1 Western Blot Antibodies
We were looking for an antibody to test the time-dependent role of a growth factor on Ref-1 protein level in mammalian smooth cells. Ref-1 is a well-known transcription factor which regulates many genes. Our antibody search was ended with the Santa Cruz Biotechnology Inc antibody, and it was inspiring to see the antibody specificity which was indicated in the flyer. Thus, to complete our goal, the log phase of growth, arrested cells were treated with specific growth factor for a different period. From these cells, the protein extract was prepared and resolved in 10 % SDS-PAGE. The resolved protein was western transferred on the nitrocellulose membrane and probed for Ref-1 using Santa Cruz Biotechnology Inc Ab.
Western Blot
Mammalian whole-cell protein lysate
Dilution (1:500) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degree Celsius
5% skimmed milk made in 1x TBST
Goat anti-mouse IgG HRP Ab, dilution (1:4000) in 5% skimmed milk made in 1x TBST, 1hr at RT
None
Enhanced chemiluminescent detection in X-Ray film
Santa Cruz Biotechnology Inc antibody has detected only a single band of Ref-1 with high specificity in the mammalian protein sample.
N/A
A specific band of ABCA1 were observed
Antibody showed a potential non-specificity
State of the art antibody for Immunodetection of Ref-1 in mammalian cells.