Great Brilliant Violet 510™ Anti-Mouse CD4 Antibody from BioLegend

October 29, 2019

Innate Immunity
DRFZ
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 510™ anti-mouse CD4 Antibody

Catalog Number:

100559

Clone Number:

RM4-5

The aim of our research is to check the different expression profiles, in terms of cytokines and surface markers, of in vitro differentiated CD4+ T cells. This antibody was used in order to identify CD4+ T cells co-cultured with APCs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

CD4+ murine T cells

Primary Incubation

15mins in 4°C

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACSymphony, software FACSDiva

Results Summary

A very good, specific antibody for CD4+ T cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good, sensitive, high resolution

The Bad

No bad things about it

The Bottom Line

I would definitely recommend it.

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