CO2 Insensitive Starvation Medium

Seoul National University
Biology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific (Gibco)

Product Name:

HBSS, calcium, magnesium, no phenol red

Catalog Number:

14025092

Good for live cell imaging under no CO2 condition.

Experimental Design and Results Summary

Application

Cell starvation especially for live cell imaging

Starting Material

Human tissue culture cell line HCT116

Tips

CO2 chamber is not required

Results Summary

I use this for live-cell imaging without the CO2 chamber. Cells looked okay and autophagy was properly induced.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Convenient, cheap, strong starvation

The Bad

None

The Bottom Line

This medium is as good as EBSS, but recommended when incubating cells in no CO2 condition.

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