Editor's Note: All antibody reviews appear in protocol format.
Primary Antibody: Rabbit anti-Pan cadherin Antibody (Whole antiserum)
Antigen/Epitope: C-terminal cadherin tail (regardless of cadherin type)
Catalog number: Sigma-Aldrich C3678
Sample: Human colon, 15 µm-thick section
Secondary Antibody: Alexa Fluor 488 Donkey anti-Rabbit IgG (H+L)
Catalog number: Life Technologies A-21206 (from Molecular Probes)
Block: 10% Normal donkey serum (NDS) in PBS
Incubation time: 60 min
Incubation temperature: 25°C
Primary Antibody Incubation
Dilution used: 1/1000
Diluent composition: PBS+ NDS 1%
Incubation time: 24 hr
Incubation temperature: 4°C
Secondary Antibody Incubation
Dilution used: 1/500
Diluent composition: PBS + NDS 1%
Incubation time: 1 hr
Incubation temperature: 25°C
Detection Method: Confocal microscope (Leica TCS SP2) observation of tissue section mounted onto a slide.
Results Summary: I observed good staining in human colon epithelium cells and in smooth muscle cells (longitudinal and circular).
Image: Transverse sections through human colon vilosities (below)
(Note: The image at the top of this page is from Sigma-Aldrich.)