Acetyl Tubulin Levels Using Anti-Alpha Tubulin Antibody

October 23, 2019

chemical biology
indian institute of chemical technology

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Company:

Abcam

Product Name:

Anti-alpha Tubulin (acetyl K40) antibody

Catalog Number:

ab24610

Clone Number:

6-11B-1

Cell lysate of Neuro2A cells treated with HDACi used to check the levels of acetyl tubulin in treated (HDACi) and control conditions.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Neuro2A (Mouse neuroblastoma) cell lysate

Primary Incubation

1:1000 in 5%BSA in 1xTBST (0.1Tween 20)

Blocking Agent

5% BSA in 1xTBST (0.1% Tween 20)

Secondary Incubation

1:10000 (Anti-mouse) in 5%BSA in 1xTBST (0.1Tween 20)

Tertiary Incubation

Not used

Detection

Chemiluminescence

Results Summary

We have observed increased acetyl tubulin levels in Neuro2A cells treated with HDACi compared to control sample.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No non specific bands observed

The Bad

Nothing bad about it

The Bottom Line

Suggest to use for checking the acetyl tubulin levels

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