MilliporeSigma
Anti-Ubiquitin Antibody, Lys48-Specific
05-1307
Apu2
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Ubiquitin is a 8.6kDa small regulatory protein present in most tissues of eukaryotic organisms and expressed ubiquitously. This protein will add to a substrate protein hence it is called ubiquitination. Ubiquitination regulates many proteins and decide the fate of a protein and mark them for degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Here we checked the time course effect of chemoattractant on Ubiquitin.
Western Blot
Mouse lysate
1:1000 dilution prepared in 1x TBST
5% milk with 1x TBST
1:4000 dilution prepared in 1x TBST
None
ECL
There is a time dependent effect of ubiquitin mediated degradation by chemoattractant.
N/A
This antibody is very good for western blot experiment
This antibody is very good for western blot to identify the lysine mediated ubquitin for any protein. Highly recommended.