This BioLegend Antibody Is Great At Staining PD-1+ T Cells

November 07, 2019

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

PE/Cy7 anti-mouse CD279 (PD-1) Antibody

Catalog Number:

109109

Clone Number:

29F.1A12

We wanted to study PD-1 expression on T cells in the diseased vs healthy state, so we used the antibody to identify PD-1+ T cells in mice.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody stains PD-1+ cells very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody clearly identifies PD-1+ cells

The Bad

None to mention

The Bottom Line

The antibody is great for identifying PD-1+ cells.

Share your experience with other scientists. Write a Review! »

Join the discussion