The Reagent Is Great for Distinguishing Live and Dead Cells

November 05, 2019

Stanford University
Pathology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation

Catalog Number:

L34975

We were studying immune cells in mice, so we used the reagent to distinguish live vs dead cells for downstream analysis.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

Splenocytes activated with anti-CD3 ex vivo

Tips

Perform the staining step prior to antibody staining and in buffer with less protein will lead to cleaner separation between live and dead.

Results Summary

The reagent is great for distinguishing live and dead cells.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

The reagent is great for separating live and dead cells

The Bad

None to mention

The Bottom Line

The reagent is great for identifying live cells for further analysis.

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